BlotCycler - Automated Western Blotting

Precision Biosystems

BlotCycler Touch is designed to automate the immunodetection step of the procedure that is traditionally performed manually.

• All steps after protein transfer to a membrane are fully automated
• The product has a total of six trays with three trays on each side 
• Processing of up to 12 membranes simultaneously with two different protocols
• Simultaneous processing of 6 different Primary and 6 different Secondary antibodies
• You can program the system by choosing your blocking time, primary antibody incubation time, secondary incubation time, and the number of washing cycles

 

The protocol involves

• Multiple solution changes and consists of blocking
• Incubation with primary and secondary antibodies
• Washing steps

 

• Patented fluidic control system combined with an automated shaking motion
• Consistent and reproducible dispensing, mixing, and distribution of the reagents throughout the process
• Automation increases productivity and generates blots with higher signal and lower background
• There’s no change in workflow. You use your existing protocols and reagents.

 

 

Precision Biosystems offers reagents

CY-BLUE: Precision Blue Film

• Optimized for use in all blue and green light chemiluminescens systems
• Coated on both sides for improved consistency and overall performance

PrecastGel 

• Polyacrylamide minigels for SDS-PAGE
• Reproducible protein separation for the analysis of protein mixtures and downstream Western blotting
• For better separation gels use Tris-glycine Laemmli electrophoresis buffers
• Gel cassettes have labeled wells for easy loading
• Compatible with multiple vertical tank electrophoresis systems

Precision Stripping Buffer

• Optimized to efficiently remove primary and secondary antibodies but leave antigen attached to the membrane
• Saves your primary antibodies.
• After running a protocol, use Precision Stripping Buffer, and you can reuse your membrane up to 10 times!

 

Documents and resources

For more information visit producer's website.