CHIP-Seq Protein-DNA Interaction

Clontech

Chromatin immunoprecipitation sequencing (ChIP-seq) has facilitated tremendous progress for epigenetics research. Given that many proteins of interest (e.g. transcription factors and modified histones) are associated with a limited number of specific genomic loci, the amount of DNA recovered by chromatin immunoprecipitation can be quite low, posing challenges for the generation of representative ChIP-seq libraries.

 

You can choose from available kits

SMARTer ThruPLEX DNA-seq Kit

• Illumina library construction
• Inputs of 50 pg – 50 ng double-stranded ChIP DNA
• Directly compatible with the DNA SMART ChIP-Seq Kit

 

DNA SMART ChIP-Seq Kit

• Highly sensitive kit generates robust sequencing libraries from low-input DNA samples
• Inputs of 100 pg – 10 ng of single or double-stranded DNA
• Ligation-free protocol
• Illumina-ready sequencing libraries
• Multiplexing of up to 48 samples
• Generates libraries in 4 hours

 

ChIP Elute Kit

• Fast (one hour) cross-link reversal and elution of ChIP DNA
• Replaces long and tedious protocols for the final stages of ChIP experiments
• ssDNA can be recovered in one hour compared to traditional methods that may require overnight incubation
• Directly compatible with the DNA SMART ChIP-Seq Kit

 

Documents and resources

For more information visit producer's website.

SELECTION GUIDE Next-Gen Sequencing Kits