Competent Cells for Cloning

TaKaRa Bio

Chemically Competent Cells

• Stellar chemically competent cells with high efficiency for cloning  
• Useful for cloning of methylated DNA (It lacks the gene cluster for cutting foreign methylated DNA)
• Can be used in wide range of applications
  • Preparation of cDNA and genomic libraries
  • Construction of long genomic libraries
  • Subcloning and methylated DNA cloning

Based on an E. coli HST04 strain that lacks the genetic factors (dam and dcm) necessary for the methylation of DNA. Plasmids prepared using this product can be cut by restriction enzymes which are normally blocked by dam or dcm methylation. A dam/recA double mutation is lethal, so the parent strain is recA+. Therefore, it follows that transformation of extracellular DNA with repeat sequences can result in recombination by recA.

 

Electrocompetent Cells

• Stellar Electrocompetent Cells offer high transformation efficiency
• Can be used in many applications (generation of cDNA libraries or constructing gene banks)
• Lack the gene cluster which digests foreign methylated DNA (mrr-hsdRMS-mcrBC and mcrA).
•  Based on the HST08 E. coli strain
• These cells paired with pUC plasmid vectors provide
  • High transformation efficiency
  • Good reproducibility
  • Blue/white screening 

 

Documents and resources

For more information visit producer's website.