His-Tagged Protein Purification

TaKaRa Bio

For your research you can choos from wade range of available kits

1) Capturem Kits

The Capturem His-Tagged Purification Miniprep Kit

• The Next Generation of His-Tagged Protein Purification
• Revolutionary solution for recombinant protein purification
• This complete system effortlessly outperforms conventional methods
• Spin columns with high-capacity membranes instead of resin
• Elute concentrated protein: up to 0.3–1 mg/ml
• Speed: 5 min at room temperature instead of 1–2 hour in the cold room
• Flexibility: purify from mammalian or bacterial cells

 

Protein purification miniprep workflow 

 

Capturem Maxiprep Kits

• Effortlessly outperforms conventional methods
• Convenient, powerful and efficient research tool
• Isolate ~ 2.5 mg of your his-tagged protein with a purification system 
• High-capacity: columns contain novel nylon membranes instead of resin
• High-yield: elute concentrated protein (up to 4.5 mg/ml)
• Fast and convenient: work for 15 min at room temperature instead of 3–4 hour in the cold room
• Flexible: purify from mammalian or bacterial cells

 

Capturem His-Tagged Purification Large Volume

• Revolutionary solution for rapidly purifying and concentrating proteins
• From large volumes of mammalian or bacterial cell lysate under a wide range of conditions
• Isolate his-tagged proteins of interest with a purification system
• Works in 10–30 min at room temperature instead of 6–10 hour in the cold room
• High yield: up to 25 mg/unit, using a novel membrane technology instead of resin
• Highly concentrated eluate: up to 2.0 mg/ml
• Flexible: purify from mammalian or bacterial cells

 

Capturem His-Tagged Purification 96

• High-throughput: Perform 96 minipreps simultaneously
• High-capacity: Each well contains novel nylon membranes instead of resin
• High-yield: Elute concentrated protein, up to 0.3–1 mg/ml
• Fast and convenient: Purify proteins in just minutes, using vacuum filtration or centrifugation
• Flexible: Purify from mammalian or bacterial cells

 

2) Cobalt columns 

TALON Single Step

• His-Tagged Protein Purification from Bacterial Cultures
• Complete extraction and purification in under 1 hour
• Purify target protein directly from bacterial culture
• Ready-to-use, prepacked columns
• Two sizes of columns available, for large and small scale purification

 

Native vs. denaturing purification procedures using TALON resin

 

 

HisTALON Superflow Cartridges

• Cobalt Resin FPLC Cartridges
• Easy-to-use prepacked 1 ml and 5 ml cartridges
• Maximize yield of biologically active protein
• Superior purity & high yields

 

HisTALON Gravity Columns

• Fast, Easy His-Tagged Protein Purification
• Easy-to-use prepacked gravity columns
• Maximize yield of biologically active protein
• Superior purity & high yields with TALON Resin

 

TALON Spin Columns

• Fast Small-Scale His-Tagged Protein Purification
• Exhibits high affinity for his-tagged proteins
• No copurification of proteins
• Resists metal leakage
• Performs well under a wide range of purification conditions
• Ready-to-use spin columns
• Each column contains 0.5 ml of resin

 

TALON Metal Affinity Resin

• Batch or Gravity Flow Purification of His-Tagged Proteins
• Exhibits high affinity for his-tagged proteins
• No copurification of proteins
• Resists metal leakage
• Performs well under a wide range of purification conditions

 

TALON CellThru

• His-Tagged Protein Purification from Crude Cell Lysates
• Purification from crude cell lysate
• Ideal for purification of membrane-bound protein or multiprotein complexes
• No copurification of proteins
• Low metal ion leakage
• Fast & easy protocol

 

TALON Superflow Metal Affinity Resin

• FPLC Purification of His-Tagged Proteins
• Exhibits high affinity for his-tagged proteins
• Ideal for FPLC applications
• No copurification of proteins
• Resists metal leakage
• Performs well under a wide range of purification condition

 

TALON Magnetic Beads

• His-Tagged High-Throughput Micro-Scale Purification
• Quick and easy separation of his-tagged proteins
• Highly selective TALON chemistry for increased purity
• Eluted samples ideal for small volume applications

 

3) Nickel Resins

His60 Ni Resin

• His-Tagged Recombinant Protein Purification
• Up to 60 mg/ml of recombinant his-tagged protein
• Low metal ion leakage in eluted his-tagged protein
• For purification under native or denaturing conditions
• Use for batch/gravity flow and automated FPLC purification

 

His60 Ni Magnetic Beads

• His-Tagged High-Throughput Microscale Purification
• Quick and easy separation of his-tagged proteins
• Selective His60 Ni chemistry for increased purity
• Eluted samples ideal for small volume applications

 

His60 Ni Superflow Cartridges

• His-tagged protein purification on any LC system
• Up to 60 mg/ml of recombinant his-tagged protein (1 ml cartridge)
• Up to 300 mg/ml of recombinant his-tagged protein (5 ml cartridge)
• Low metal ion leakage in eluted his-tagged protein
• For purification under native or denaturing conditions
• Fast and reproducible processing on any LC system, including ÄKTA

 

His60 Ni Gravity Flow Columns

• His-Tagged Recombinant Protein Purification
• Obtain up to 60 mg/ml of recombinant his-tagged protein
• Low metal ion leakage in eluted his-tagged protein
• Purify under native or denaturing conditions
• Use for batch/gravity flow and automated FPLC purification

 

Documents and resources

For more information visit producer's website.