Immune Repertoire Profiling

Clontech

SMARTer Human and Mouse TCR a/b Profiling Kits

• Kits provide complete V(D)J sequence information using NGS and 5' RACE-like approach
• Customer obtains full-length sequences for variable regions of TCR mRNA transcripts
• Profiling diversity for α and β TCR subunits, either in the same experiment or separately
• Sample input: RNA obtained from peripheral blood, or from purified T-cells
• Amplification of sequences for each TCR subunit with a single primer pair per reaction
• Kit incorporates Illumina adapter and index sequences in a ligation
• High sensitivity – detection of low-abundance TCR sequences, even with shallow read depths
• Excellent reproducibility – generation of consistent results across a range of input amounts

 

You can choose from these type of kits:

SMARTer Human scTCR a/b Profiling Kit - for single T-cells

SMARTer Human TCR a/b Profiling Kit - 10 ng -3 µg of total RNA or 50-10.000 purified T-cells

SMARTer Mouse scTCR a/b Profiling Kit - 10 ng - 500 ng total RNA or 1.000-10.000 purified T-cells

 

SMARTer Human BCR IgG IgM H/K/L Profiling Kit

• Sensitive and accurate human BCR repertoire analysis of heavy and light chains
• Total RNA inputs: 10ng - 1 µg of RNA from blood or 1- 100 ng of RNA from B-cells
• Kit pairs SMARTer 5’-RACE chemistry to capture the entire V(D)J region with NGS
• Complete wokflow includes cDNA synthesis, targeted amplification and software
• Takara Bio Immune Profiler Software for  sequencing data analysis
• Flexible pooling strategy for highly sensitive detection of different chains from the same sample
• UMI-based error correction - removing of reads derived from PCR errors or duplication/sequencing errors

 

SMARTer Mouse BCR IgG H/K/L Profiling Kit

• Highly sensitive and reproducible method for profiling B-cell repertoires
• Accurate determination of the clonotypes and isotypes (specific H, K, and L gene segments)
• Input:10 ng - 3µg of total RNA or 50 - 10.000 purified B-cells
• Kit pairs 5’ RACE with NGS to provide a sensitive, accurate approach to BCR profiling
• Gene-specific amplification to capture complete V(D)J variable regions of BCR transcripts
• Sequencing of H/K/L chains for accurate identification of clonotypes and reliable assignment of isotype

 

Documents and resources

For more information about visit producer's website.

Takara Bio Immune Profiler Software

SELECTION GUIDE Next-Gen Sequencing Kits