In-Fusion Cloning Kits

TaKaRa Bio

• Kits need ligation and are absolutely seamless
• Final construct have no extra base pairs left over
• The reaction last 15 minutes for single or multiple fragments
• Insert cloning yields are over 90 %
• Over 95% efficiency is demonstrated with a broad range of fragment sizes from 0,5-15 kb
• The large inserts of vectors are directly clone into the expression plasmid

 

You can choose form available kits

In-Fusion HD Liquid Kits

In-Fusion HD EcoDry Kits

• Pre-aliquoted, lyophilized components that minimize handling errors
• Components can be stored at room temperature eliminating freeze-thaw cycles

 

Applications

• PCR Cloning
• HTP Cloning
• Directional cDNA cloning
• Mutation generation
• Multiple fragment cloning
• Adding adaptors, linkers, protein tags
• Cloning using modular parts
• High-throughput cloning
• Gene synthesis
• Gene design
• Site-directed mutagenesis
• Domain swapping
• Domain modification

 

Documents and resources

For more information visit producer's website.