Single Cell and Ultra-Low-Input RNA-Seq

Clontech

Robust and sensitive enough NGS library preparation methods for reproducibly capture RNA molecules present in minute quantities.

 

cDNA SYNTHESIS FROM SINGLE CELL

SMART-seq Single Cell Kit

• Uses robust chemistry for single-cell RNA analysis with ultimate sensitivity
• Generates high-quality, full-length cDNA directly from different single cell types
• It has been validated with 2 pg of total RNA input
• Good results with low RNA content cells like peripheral blood mononuclear cells
• cDNA is compatible with lllumina platform-specific library preparation kits
• Easy, plate-based workflow - direct input of single cells isolated by FACS or other methods
• Highly scalable workflow - high-throughput capabilities and automation-friendly
• Compatible with droplet-based technologies, such as 10X Genomics

 

cDNA SYNTHESIS FROM ULTRA-LOW-INPUT RNA

• cDNA synthesis from 1 - 1,000 cells, or 10 pg –10 ng high-quality (RIN>8) total RNA
• Kits use oligo(dT) priming
• Rely on proprietary SMART (Switching Mechanism at 5’ end of RNA Template) technology
• SMART technology ensures full-length unbiased mRNA coverage
• Powerful approach for resolving biologically relevant differences between individual cells
• Analysis of rare or precious samples (stem cells, circulating tumor cells and tissue biopsies)
• Improved chemistry relative to previous versions and locked nucleic acids (LNA)-enhanced oligos
• Accurate representation of GC-rich transcripts
• Compatible with Illumina or Ion Torrent sequencing platforms

 

You can choose from available kits

1) Full-length cDNA

SMART-Seq HT Kit

• for high-throughput applications
• cDNA synthesis from 1 - 100 cells or 10 pg –1 ng total RNA

 

SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing

• cDNA synthesis from 1 - 1,000 cells or 10 pg –10 ng total RNA

 

SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System

• cDNA synthesis from single cells

 

2) 3'​ end only

SMART-Seq v4 3'​ DE Kit

• cDNA synthesis from 1 - 100 cells or 10 pg –1 ng total RNA
• Compatible  with Illumina library prep only

 

 

LIBRARY PREPARATION

SMART-Seq Stranded Kit

• Illumina ready library generation from 1-1000 cells or 10 pg-10 ng of total RNA
• For RNA of any integrity (RIN 2-10)
• Streamlined workflow: 7 hours from sample to sequencer
• Unparalleled sensitivity for single-cell, full-length total RNA sequencing 
• Retains strand of origin information
• Incorporated rRNA depletion step using ZapR and R-Probes optimized for single-cell
• Uses a random-priming approach to capture the entire transcriptome
• Product is available in 3 sizes of 12 rxns, 48 rxns and 96 rxns

 

 

Documents and resources

For more information about SMART-seq Single Cell Kit visit producer's website.

For more information about cDNA synthesis visit producer's website.

For more information about SMART-Seq Stranded Kit visit producer's website.

SELECTION GUIDE Next-Gen Sequencing Kits