Single-Cell Whole Genome Amplification

TaKaRa Bio

The PicoPLEX Kits with patented technology is designed and optimized for amplification of single-copy genomic DNA starting with a single cell. The easy-to-use protocol reduces handling errors, dramatically improves time to results, and reduces background. Solutions for both NGS and PCR/array-based approaches.

 

1) PicoPLEX Gold Single Cell DNA-Seq Kit

Kit is designed to generate high-quality DNA libraries from single cells for sequencing on Illumina platforms. The kit is based on patented PicoPLEX technology and is optimized for the reproducible detection of copy number variants (CNVs), single nucleotide variants (SNVs), indels, and small structural variants. The PicoPLEX Gold kit outperforms the leading Multiple Displacement Amplification (MDA) technology for SNV detection.

• Illumina-ready sequencing libraries
• Inputs of 1-10 mammalian cells or 6-50 pg gDNA
• Highly reproducible and accurate CNV, SNV, and aneuploidy detection
• Outperforms the leading MDA technology for SNV and CNV detection
• Streamlined three-hour workflow with less than 30 minutes of hands-on time
• Four-step, two-tubes protocol 
• Highly uniform coverage with low allele drop-in and drop-out rate

 

 

2) PicoPLEX Single Cell WGA Kit v3

This kit uses technology designed and optimized for the amplification of single-copy genomic DNA starting from a single cell. Accepted for use in array CGH analysis as well as PCR. Excellent for detection of copy number variants (CNVs).

• Amplicon enrichment panels, hybridization-based enrichment, NGS library prep
• qPCR assays to detect SNV
• Array comparative genomic hybridization (aCGH) and SNP genotyping
• Amplify DNA from 1-10 cells, or from as little as 6 picograms of DNA
• Superior reproducibility of allele representation
• Streamlined and fast single tube workflow
• Sample to library in ~2.5 hours

 

SMARTer DNA indexes Kits

• Contain PCR primers for the amplification of Illumina-compatible NGS libraries
• Can be used with all of TaKaRa Bio's DNA-seq kits to generate libraries
• Both single and dual indexes available
• Indexes are sold separately

 

 

Documents and resources

For more information about PicoPLEX Gold Single Cell DNA-Seq Kit visit producer's website.

For more information about PicoPLEX Single Cell WGA Kit v3 visit producer's website.

SMARTer DNA indexes Kits

Downlands SELECTION GUIDE Next-Gen Sequencing Kits