SMARTer ICELL8 Single-Cell Analysis Systems
TaKaRa Bio
Unparalleled single-cell analysis including isolation and selection of cells and sample throughput
Takara Bio offers:
1) SMARTer ICELL8 cx Single-Cell System
- compact version of the original ICELL8 for single cell sequencing automation
2) SMARTer ICELL8 Single-Cell System
- original system, consists of several separate parts
ICELL8 cx and ICELL8 Systems Features:
- Evaluation of up to 8 sample types or conditions on each chip
- Unbiased isolation of 1,000–1,800 single cells in a 5,184-nanowell chip
- Maximizing of throughput with rapid dispensing and imaging
- Analysis of cells with sizes of 5-100µm with unbiased cell isolation
- Isolation of various cells (e.g. nuclei, cardiomyocytes, B lymphocytes, neurons, spheroids, etc.)
- Imaging to differentiate single from multiple cells and perform live/dead analysis with fluorescence
- Multiple imaging channels (red, blue, green)
- Choice of specific cells to process using advanced imaging software
- Maximising experimental efficiency by analysing only cells of interest
- Reagents optimised to enhance cell separation and viability
- Blank chips and preprinted chips for specific applications
- Flexibility to use pre-validated SMARTer NGS chemistries or developped custom applications
MultiSample NanoDispenser (MSND)
- Deposits nanoliter volumes of cells and reagents into the ICELL8 Chip
- Capable of dispensing 8 unique samples evenly across the chip in 15 minutes
- Rapid dispensing helps to ensure cell viability throughout the cell isolation process
- Temperature and humidity controlled environment
Imaging Station
- Easy to use optical system, purpose-configured for the ICELL8 Chip
- Rapid image capture of individual wells on the chip
- Includes fluorescence detection of commonly used dyes in single cell research
- Designed to interface directly with CellSelect software
CellSelect Software
- Interrogates images of each well
- Evaluates parameters to determine the number of cells present and the cell viability state
- Identify wells with single cells and evaluate viability to recommend wells for downstream processing
Applications
- Differential expression by 3' end counting
- TCR profiling and 5′ differential expression
- Full-length transcriptome analysis
- ATAC-seq
1) SMARTer ICELL8 cx Single-Cell System
Full system includes:
- SMARTer ICELL8 cx Thermal Cycler
- Built-in imaging capabilities
- Integrated SMARTer ICELL8 cx CellSelect Software
- Advanced, integrated automation platform
- End-to-end automation for dispensing and isolating single cells in 5,184-nanowell chips
- Large-bore nozzle for unbiased cell size isolation
2) SMARTer ICELL8 Single-Cell System
System consists of:
- MultiSample NanoDispenser (MSND)
- Imaging Station
- CellSelect Software
Documents and resources
For more information visit producer's website.
SMARTer ICELL8 cx Single-Cell System Video
SMARTer ICELL8 cx Single-Cell System User Manual
SMARTer ICELL8 cx CellSelect Software User Manual
SMART-Seq ICELL8 cx Application Kit User Manual
ICELL8 MultiSample NanoDispenser User Manual
ICELL8 Imaging System User Manual
CellSelect Software User Manual
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